Volcanic rocks from ophiolitic formations in northeast Othris region are categorized based on their geochemical characteristics in two distinct groups. The first includes volcanic rocks from the ophiolitic formations of Eretria and Velestino, which, as their immobile element chemistry and geochemical plots indicate, seem to have formed in an N-MORB environment with 5-15% partial melting of a fertile or moderately depleted mantle source and extensive fractional crystallization processes. The second group is exclusively from the ophiolitic formation of Aerino having rocks with generally higher MgO contents, subduction related features (e.g. low Ti/V<10) and having been derived from a highly depleted mantle source but with similar partial melting degrees (10-20%). These differences may reflect an evolution from an earlier MORB to a latter IAT volcanism within the same oceanic basin or correspond to two separate oceanic environments.